An assessment of high-pressure freezing and freeze substitution protocols for cultured cells
نویسندگان
چکیده
Since the advent of cryo-electron microscopy (cryo-EM), the close-to-life and fine structure preservation of biological samples has become a realized procurement, surpassing the fixation quality and avoiding the numerous artifacts inherent to conventional chemical preservation methods. Cryo-EM techniques such as high-pressure freezing followed by freezesubstitution (HPF/FS) have facilitated the simultaneous immobilization of all supramolecular complexes within a sample within milliseconds, providing a true snapshot of the cell at the moment of freezing.
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